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Team:Warsaw/Calendar-Main/1 July 2008
From 2008.igem.org
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<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pET15b plasmid.</li> | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pET15b plasmid.</li> | ||
| - | <li> Overnight <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of pET15b plasmid with NdeI and BamHI, <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation with CIAP</a>.</li></ol></p> | + | <li> Overnight <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of pET15b plasmid with NdeI and BamHI (Tango 2x buffer), <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation with CIAP</a>.</li></ol></p> |
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Revision as of 13:53, 11 October 2008
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Cloning alpha-A and omega-A fusions on pKSMichał L., Ewa, Marcin
We have successfully cloned A-alpha and A-omega fusions on pKS vector. The DNA will be now sequenced to ensure that our constructs are correct. Preparation of constructs with OmpA protein fusionsPaweł
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