Team:Warsaw/Calendar-Main/24 July 2008

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<ol><li>Separate transformant colonies (tranformation of ligation of pACYC177+OmpA_alpha and omega_A from previous day) inoculated to liquid LB with kanamycin.  
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<li>Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega. Primers used:
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a></li>
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<li>Confirmed transformant colonies inoculated to liquid LB with kanamycin. </li>
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Revision as of 20:38, 11 October 2008

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Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA

Paweł

  1. Results of ligation: 6 colonies grown.
  2. Each colony cultured overnight in LB + ampicillin.
  • Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega. Primers used: AL+SacI and AP+NotI
  • Confirmed transformant colonies inoculated to liquid LB with kanamycin.

    • Antoni:

    Preparation of chemocompetent bacteria E. coli TOP10.

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