From 2008.igem.org
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| - | <h3>Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>)</h3>
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| - | <h4>Piotr</h4>
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| - | <p>
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| - | Test was conducted in <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain expressing omp_omega_A_alfa (with and without induction) and omp_A_alfa.
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| - | <ol>
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| - | <li>Spinnign</li>
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| - | <li>Suspending</li>
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| - | <li>Adding of lysis buffer</li>
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| - | <li>Boiling</li>
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| - | <li>Putting into poliacrylamide gel</li>
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| - | <li>Transfer onto nitrocellulose</li>
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| - | <li>Blocking</li>
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| - | <li>Anti-A antibody binding</li>
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| - | <li>Washing</li>
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| - | <li>Anti-rabbit antibody binding</li>
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| - | <li>Developing with BCIP and NBT</li>
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| - | </ol>
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| - | </p>
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| - | [photo of the gel is to be placed here]
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| - | <p>We didn't observe differences in espression and degradation in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosettas</a> nor in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>. Therefore we suppose that degradation of the fusions is caused by other factor than Lon and OmpT proteases.</p>
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Revision as of 00:06, 12 October 2008
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