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Team:Warsaw/Calendar-Main/20 June 2008
From 2008.igem.org
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from cultures inoculated on previous day.</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from cultures inoculated on previous day.</li> | ||
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with PstI (Orange buffer).</li> | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with PstI (Orange buffer).</li> | ||
| - | <li>Gel electrophoresis (no proper colonies founded).</li></ol> | + | <li>Gel electrophoresis (Fig. 1 and 2, no proper colonies founded).</li></ol> |
<h3>Preparation of alpa-A and omega-A fusions</h3> | <h3>Preparation of alpa-A and omega-A fusions</h3> | ||
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<tr><td>4°C</td><td>infinite</td></tr> | <tr><td>4°C</td><td>infinite</td></tr> | ||
</table> | </table> | ||
| + | <br> | ||
| + | <img src="https://static.igem.org/mediawiki/2008/7/78/Omp_control_digest_WAW.jpg" width=400/> | ||
| + | <var>Fig. 1. Lanes 1 to 8 - control digests with PstI of 8 clones with probably alpha_OmpA fusion.</var> | ||
| - | + | <img src="https://static.igem.org/mediawiki/2008/1/1a/Omp_control_digest2_WAW.jpg" width=400/> | |
| + | <var>Fig. 2. Lanes 1 to 8 - control digests with PstI of 8 clones with probably omega_OmpA fusion.</var> | ||
</html> | </html> | ||
Revision as of 01:17, 12 October 2008
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Preparation of constructs with OmpA protein fusionsMichał K.
Preparation of alpa-A and omega-A fusionsMichał L., Ewa, Marcin
Fig. 1. Lanes 1 to 8 - control digests with PstI of 8 clones with probably alpha_OmpA fusion.
Fig. 2. Lanes 1 to 8 - control digests with PstI of 8 clones with probably omega_OmpA fusion.
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