Team:Paris/Modeling/f3bis

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[[Image:f3env.png|thumb]]
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[[Image:f3env.png|thumb]] (see [[Team:Paris/Modeling/Oscillations#Biochemical_Assumptions|the considerations on the use of EnvZ]]
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We have [EnvZ] = {coef<sub>env</sub>}''expr(pTet)'' = {coef<sub>env</sub>} &#131;1([aTc]<sub>i</sub>)
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We have [EnvZ]<sub>produced</sub> = {coef<sub>env</sub>}''expr(pTet)'' = {coef<sub>env</sub>} &#131;1([aTc]<sub>i</sub>)
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and [EnvZ]<sub>total</sub> = [EnvZ]<sub>basal</sub> + [EnvZ]<sub>produced</sub>
and [FliA] = {coef<sub>FliA</sub>}''expr(pBad)'' = {coef<sub>FliA</sub>} &#131;2([arab]<sub>i</sub>)
and [FliA] = {coef<sub>FliA</sub>}''expr(pBad)'' = {coef<sub>FliA</sub>} &#131;2([arab]<sub>i</sub>)

Revision as of 08:39, 16 October 2008

F3env.png
(see the considerations on the use of EnvZ

We have [EnvZ]produced = {coefenv}expr(pTet) = {coefenv} ƒ1([aTc]i)

and [EnvZ]total = [EnvZ]basal + [EnvZ]produced

and [FliA] = {coefFliA}expr(pBad) = {coefFliA} ƒ2([arab]i)

So, if we denote phosphorylated OmpR by OmpR*, we have

F3ompfromenv.jpg

that we can then introduce in the previous expression (ƒ 3) :

F3ompfinal.jpg



param signification unit value
[expr(pFlhDC)] expression rate of
pFlhDC with RBS E0032
nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ratio between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
β12bis production rate of FliA-pFlhDC with RBS E0032
replace β12 (not written)
nM.min-1
(K11bis/{coeffliA}) activation constant of FliA-pFlhDC
K11bis
nM
n11bis complexation order of FliA-pFlhDC
replace n11 (not written)
no dimension
β2bis production rate of EnvZ-pFlhDC with RBS E0032
β2bis
nM.min-1
(K19bis/{coefenvZ}) activation constant of EnvZ-pFlhDC
replace K19 (not written)
nM
n19bis complexation order of EnvZ-pFlhDC
n19bis
no dimension



Then, if we have time, we want to verify the expected relation

SumpFlhDC2.jpg