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Team:Hawaii/Milestone
From 2008.igem.org
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| - | {{Team:Hawaii/Header}} | + | {{Team:Hawaii/Header}}[[Image:20080612-work breakdown structure.jpg|thumb|300px|right]] |
== Immediate == | == Immediate == | ||
<onlyinclude> | <onlyinclude> | ||
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* 6/12/08 Design and order primers | * 6/12/08 Design and order primers | ||
* 6/12/08 Make list of BioBrick parts needed for Part B | * 6/12/08 Make list of BioBrick parts needed for Part B | ||
| - | * Culturing Protocol Optimization (KS | + | * Culturing Protocol Optimization: CO2 is bottle neck, need incubator retrofitted (KS) |
| - | + | ||
* Compare RP4 sequence with OriT (MR) | * Compare RP4 sequence with OriT (MR) | ||
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</onlyinclude> | </onlyinclude> | ||
| - | + | == Near Term == | |
| - | == | + | |
* [[Team:Hawaii/Meeting/2008-06-27|2008.06.27]] Make final decision on Part B of our iGEM project | * [[Team:Hawaii/Meeting/2008-06-27|2008.06.27]] Make final decision on Part B of our iGEM project | ||
| - | * | + | * RSF1010 (pRL1383a) MCS Replaced |
== Completed Milestones == | == Completed Milestones == | ||
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* 2008.06.03-2008.06.04 Plasmid Transformation, document results (GK/NW) | * 2008.06.03-2008.06.04 Plasmid Transformation, document results (GK/NW) | ||
* 2008.06.04 GK and KS are now autoclave trained | * 2008.06.04 GK and KS are now autoclave trained | ||
| - | * Fluorometer found | + | * 2008.06.10 Fluorometer found (in Dr. Su's lab), 470nm blue LED lights purchased for constructing our own light table. |
| + | * 2008.06.11 First draft Work Breakdown Structure diagram drawn on whiteboard, detailing all deliverables. (please keep it updated) | ||
| + | * 2008.06.11 Does RSF1010 replicate autonomously in PCC6803? YES, only when it goes in cell as circle. linear DNA need recombination. Determined we need to test all three methods to find one that works: 1. Chemical 2. Electroporation 3. Conjugation (MR) | ||
{{Team:Hawaii/Footer}} | {{Team:Hawaii/Footer}} | ||
Revision as of 01:31, 13 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Immediate
- 6/12/08 Final draft of proposal due
- 6/12/08 Design and order primers
- 6/12/08 Make list of BioBrick parts needed for Part B
- Culturing Protocol Optimization: CO2 is bottle neck, need incubator retrofitted (KS)
- Compare RP4 sequence with OriT (MR)
Near Term
- 2008.06.27 Make final decision on Part B of our iGEM project
- RSF1010 (pRL1383a) MCS Replaced
Completed Milestones
- 2008.05.xx figure out OD Reading operation: Beckman 640U spectrophotometer (AB/NW)
- 2008.05.24 Create seed stocks for test transformation (NW/MR)
- 2008.05.22 Describe, present [http://partsregistry.org/Part_Types part types]. 1. Norman: biobrick assembly, parts, 2. Margaret: devices, 3. Krystle: chassis & plasmids, 4. Grace: other
- 2008.06.01 Plasmid Transformation Plan/Protocol (GK/MR)
- 2008.06.02 Create competent DH5a E. coli stocks (GK/MR/NW)
- 2008.06.03-2008.06.04 Plasmid Transformation, document results (GK/NW)
- 2008.06.04 GK and KS are now autoclave trained
- 2008.06.10 Fluorometer found (in Dr. Su's lab), 470nm blue LED lights purchased for constructing our own light table.
- 2008.06.11 First draft Work Breakdown Structure diagram drawn on whiteboard, detailing all deliverables. (please keep it updated)
- 2008.06.11 Does RSF1010 replicate autonomously in PCC6803? YES, only when it goes in cell as circle. linear DNA need recombination. Determined we need to test all three methods to find one that works: 1. Chemical 2. Electroporation 3. Conjugation (MR)
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
