Team:Hawaii/Notebook/2008-10-16

From 2008.igem.org

(Difference between revisions)
(→Verification of transformants)
(margaret)
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:* Colony PCR of transformants
:* Colony PCR of transformants
::* None of the colonies tested positive for their desired inserts
::* None of the colonies tested positive for their desired inserts
 +
 +
===Transformation===
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:<strong> Margaret</strong>
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{|class=wikitable border=1 align=center
 +
!Construct
 +
!Colonies
 +
|-
 +
|align=center|lac promoter/rbs(34)+ rep + pSB1A2
 +
|align=center|0
 +
|-
 +
|align=center|lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (A) +pSB1A3
 +
|align=center|113
 +
|-
 +
|align=center|lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (A)
 +
|align=center|141
 +
|-
 +
|align=center|lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (B) +pSB1A3
 +
|align=center|150
 +
|-
 +
|align=center|oriV (6) + oriT + pSB1A7
 +
|align=center|200
 +
|-
 +
|align=center|pSB1A7 self (did not de-phosphorylate)
 +
|align=center|50
 +
|-
 +
|align=center|DH5-a with RP4
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|align=center|3
 +
|-
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|align=center|DB3.1 with RP4
 +
|align=center|0
 +
|-
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|align=center|(+) pUC18
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|align=center|lawn
 +
|-
 +
|align=center|(-) no plasmid
 +
|align=center|0
 +
|}
 +
= Discussion =
= Discussion =

Revision as of 03:25, 19 October 2008

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Things we did today

Wetlab work

Verification of transformants

Grace
EtBr stained 2% agarose gel ran at 95V for 1 hour.
Construct Colonies
nir + rbs+GFP+tt in BBpRL1383a-1 2
plac + rbs+GFP+tt in BBpRL1383a-1 0
J33207 in pRL1383a (all plates) lawn
  • Colony PCR of transformants
  • None of the colonies tested positive for their desired inserts

Transformation

Margaret
Construct Colonies
lac promoter/rbs(34)+ rep + pSB1A2 0
lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (A) +pSB1A3 113
lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (A) 141
lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (B) +pSB1A3 150
oriV (6) + oriT + pSB1A7 200
pSB1A7 self (did not de-phosphorylate) 50
DH5-a with RP4 3
DB3.1 with RP4 0
(+) pUC18 lawn
(-) no plasmid 0


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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