Team:Warsaw/Calendar-Main/11 June 2008

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<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a>.</li></ul>
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a>.</li></ul>
<li>Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</li>
<li>Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</li>
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<li>Plating transformants on liquid LB + Tetracycline + Ampicillin 30 μg/mL</li>
+
<li>Plating transformants on liquid LB + Tetracycline + Ampicillin 30 μg/mL + 0.1% Ara</li>

Revision as of 13:21, 19 October 2008

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Blue/white and rifampicin test

Michał L., Ewa, Piotr

  1. Transformation of E. coli TOP10 carrying pZC320 with:
  2. Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).
  3. Plating transformants on liquid LB + Tetracycline + Ampicillin 30 μg/mL + 0.1% Ara

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