Imperial College/15 October 2008

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Revision as of 14:17, 19 October 2008

August
M	T	W	T	F	S	S
				[http://2008.igem.org/Imperial_College/1_August_2008 1]	[http://2008.igem.org/Imperial_College/2_August_2008 2]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/3_August_2008&action=edit 3]
[http://2008.igem.org/Imperial_College/4_August_2008 4]	[http://2008.igem.org/Imperial_College/5_August_2008 5]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/6_August_2008&action=edit 6]	[http://2008.igem.org/Imperial_College/7_August_2008 7]	[http://2008.igem.org/Imperial_College/8_August_2008 8]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/9_August_2008&action=edit 9]	[http://2008.igem.org/Imperial_College/10_August_2008 10]
[http://2008.igem.org/Imperial_College/11_August_2008 11]	[http://2008.igem.org/Imperial_College/12_August_2008 12]	[http://2008.igem.org/Imperial_College/13_August_2008 13]	[http://2008.igem.org/Imperial_College/14_August_2008 14]	[http://2008.igem.org/Imperial_College/15_August_2008 15]	[http://2008.igem.org/Imperial_College/16_August_2008 16]	[http://2008.igem.org/Imperial_College/17_August_2008 17]
[http://2008.igem.org/Imperial_College/18_August_2008 18]	[http://2008.igem.org/Imperial_College/19_August_2008 19]	[http://2008.igem.org/Imperial_College/20_August_2008 20]	[http://2008.igem.org/Imperial_College/21_August_2008 21]	[http://2008.igem.org/Imperial_College/22_August_2008 22]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/23_August_2008&action=edit 23]	[http://2008.igem.org/Imperial_College/24_August_2008 24]
[http://2008.igem.org/Imperial_College/25_August_2008 25]	[http://2008.igem.org/Imperial_College/26_August_2008 26]	[http://2008.igem.org/Imperial_College/27_August_2008 27]	[http://2008.igem.org/Imperial_College/28_August_2008 28]	[http://2008.igem.org/Imperial_College/29_August_2008 29]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/30_August_2008&action=edit 30]	[http://2008.igem.org/Imperial_College/31_August_2008 31]

September
M	T	W	T	F	S	S
[http://2008.igem.org/Imperial_College/1_September_2008 1]	[http://2008.igem.org/Imperial_College/2_September_2008 2]	[http://2008.igem.org/Imperial_College/3_September_2008 3]	[http://2008.igem.org/Imperial_College/4_September_2008 4]	[http://2008.igem.org/Imperial_College/5_September_2008 5]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/6_September_2008&action=edit 6]	[http://2008.igem.org/Imperial_College/7_September_2008 7]
[http://2008.igem.org/Imperial_College/8_September_2008 8]	[http://2008.igem.org/Imperial_College/9_September_2008 9]	[http://2008.igem.org/Imperial_College/10_September_2008 10]	[http://2008.igem.org/Imperial_College/11_September_2008 11]	[http://2008.igem.org/Imperial_College/12_September_2008 12]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/13_September_2008&action=edit 13]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/14_September_2008&action=edit 14]
[http://2008.igem.org/Imperial_College/15_September_2008 15]	[http://2008.igem.org/Imperial_College/16_September_2008 16]	[http://2008.igem.org/Imperial_College/17_September_2008 17]	[http://2008.igem.org/Imperial_College/18_September_2008 18]	[http://2008.igem.org/Imperial_College/19_September_2008 19]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/20_September_2008&action=edit 20]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/21_September_2008&action=edit 21]
[http://2008.igem.org/Imperial_College/22_September_2008 22]	[http://2008.igem.org/Imperial_College/23_September_2008 23]	[http://2008.igem.org/Imperial_College/24_September_2008 24]	[http://2008.igem.org/Imperial_College/25_September_2008 25]	[http://2008.igem.org/Imperial_College/26_September_2008 26]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/27_September_2008&action=edit 27]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/28_September_2008&action=edit 28]
[http://2008.igem.org/Imperial_College/29_September_2008 29]	[http://2008.igem.org/Imperial_College/30_September_2008 30]

October
M	T	W	T	F	S	S
		[http://2008.igem.org/Imperial_College/1_October_2008 1]	[http://2008.igem.org/Imperial_College/2_October_2008 2]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/3_October_2008&action=edit 3]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/4_October_2008&action=edit 4]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/5_October_2008&action=edit 5]
[http://2008.igem.org/wiki/index.php?title=Imperial_College/6_October_2008&action=edit 6]	[http://2008.igem.org/Imperial_College/7_October_2008 7]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/8_October_2008&action=edit 8]	[http://2008.igem.org/Imperial_College/9_October_2008 9]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/10_October_2008&action=edit 10]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/11_October_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/12_October_2008&action=edit 12]
[http://2008.igem.org/Imperial_College/13_October_2008 13]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/14_October_2008&action=edit 14]	[http://2008.igem.org/Imperial_College/15_October_2008 15]	[http://2008.igem.org/Imperial_College/16_October_2008 16]	[http://2008.igem.org/Imperial_College/17_October_2008 17]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/18_October_2008&action=edit 18]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/19_October_2008&action=edit 19]
[http://2008.igem.org/wiki/index.php?title=Imperial_College/20_October_2008&action=edit 20]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/21_October_2008&action=edit 21]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/22_October_2008&action=edit 22]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/23_October_2008&action=edit 23]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/24_October_2008&action=edit 24]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/25_October_2008&action=edit 25]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/26_October_2008&action=edit 26]
[http://2008.igem.org/wiki/index.php?title=Imperial_College/27_October_2008&action=edit 27]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/28_October_2008&action=edit 28]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/29_October_2008&action=edit 29]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/30_October_2008&action=edit 30]	[http://2008.igem.org/wiki/index.php?title=Imperial_College/31_October_2008&action=edit 31]

Wet Lab

Digestion of mini's

Today mini preps were prepared. These were digested and run on a gel to confirm the presence of the correct constructs. The folowing constructs were mini preped; Gene art 14 (pXyl-spoVG) and 5' EpsE integration sites. To test the correct size insert is present the mini's were digestd with EcoRI and PstI. The image below shows the results:

Several of construct 14 have appeared to have succssfully been ligated. In addition all of the 5'EpsE appear to have worked.

To construct 5'EpsE integration site we used a PCR product and as a result of the approach there is a possibility that the orientation of this biobrick in the vector is incorrect. Digestion with XbaI SpeI will confirm correct orientation.

@@ Line 9: / Line 9: @@
 ===Wet Lab===
+====Digestion of mini's====
 *Today mini preps were prepared. These were digested and run on a gel to confirm the presence of the correct constructs. The folowing constructs were mini preped; Gene art 14 (pXyl-spoVG) and 5' EpsE integration sites. To test the correct size insert is present the mini's were digestd with EcoRI and PstI. The image below shows the results:
 [[Image:Wednesday15thoct.PNG|center|400px]]
+*Several of construct 14 have appeared to have succssfully been ligated. In addition all of the 5'EpsE appear to have worked.
+*To construct 5'EpsE integration site we used a PCR product and as a result of the approach there is a possibility that the orientation of this biobrick in the vector is incorrect. Digestion with XbaI SpeI will confirm correct orientation.