"
Team:Warsaw/Calendar-Main/8 August 2008
From 2008.igem.org
(Difference between revisions)
PPrzanowski (Talk | contribs) |
|||
| Line 12: | Line 12: | ||
| + | <h3>Purification of proteins: Z-alpha and Z-omega</h3><h4>Emilia</h4> | ||
| + | |||
| + | <p>100 ml of medium inoculated with overnight culture, then used as inoculum for 1 l culture with presence of ampicillin, kanamycin and IPTG (according to <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o>protocol</a>).</p> | ||
| + | <br> | ||
Revision as of 21:11, 19 October 2008
 |
|
||||||||
 |
|
||||||||
Checking if degradation of fusion with OmpA is a result of Top10 proteases' activity (lon, ompt)Piotr:Inoculation of OmpA_omega_A_alpha and OmpA_A_alpha with and without inductor (0,5 mm/mL IPTG) in E. coli Rosetta strain. Purification of proteins: Z-alpha and Z-omegaEmilia100 ml of medium inoculated with overnight culture, then used as inoculum for 1 l culture with presence of ampicillin, kanamycin and IPTG (according to protocol).
|
|
