Brown: Team Resistance/18 October 2008

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== 18 October 2008 ==
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*Our new pvj4 primers arrived!  We will be doing a PCR of our SWT and S105 genes with their specific Prefix primers and the new pvj4 suffix primer so that we can get the entire S, R, Rz cassette rather than the single S gene as we had done before.  
*Our new pvj4 primers arrived!  We will be doing a PCR of our SWT and S105 genes with their specific Prefix primers and the new pvj4 suffix primer so that we can get the entire S, R, Rz cassette rather than the single S gene as we had done before.  
*Cultures made of our ligation transformation of S105 gene so that a verification PCR can be done before sending in.
*Cultures made of our ligation transformation of S105 gene so that a verification PCR can be done before sending in.

Revision as of 01:28, 21 October 2008


Toxipop lab notebook.png

18 October 2008

  • Our new pvj4 primers arrived! We will be doing a PCR of our SWT and S105 genes with their specific Prefix primers and the new pvj4 suffix primer so that we can get the entire S, R, Rz cassette rather than the single S gene as we had done before.
  • Cultures made of our ligation transformation of S105 gene so that a verification PCR can be done before sending in.
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