Brown: Team Resistance/21 October 2008

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(New page: {{BNBK}} ==21 October 2008== *Optical Density S105 gene Restriction Digest with EcoRI and Spe I: 116.4 ng/ul *Optical Density S105 cassette after PCR Purification: 108.6 ng/ul *Optical D...)
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Revision as of 14:39, 21 October 2008


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21 October 2008

  • Optical Density S105 gene Restriction Digest with EcoRI and Spe I: 116.4 ng/ul
  • Optical Density S105 cassette after PCR Purification: 108.6 ng/ul
  • Optical Density PVJ4 cassette after PCR Purification: 45.4 ng/ul

Restriction Digest of S105 cassette and PVJ4 cassette with EcoRI and SpeI:

  • S105

1 37.5 ul Milli-Q Water 2 5 ul Multicore 3 .5 ul BSA 4 5 ul DNA 5 1 ul EcoRI and 1 ul Spe I

  • PVJ4

1 30.5 ul Milli-Q Water 2 5 ul Multicore 3 .5 ul BSA 4 12 ul DNA 5 1 ul EcoRI and 1 ul Spe I

  • 50 ul total reaction volume
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