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Brown: Team Resistance/21 October 2008
From 2008.igem.org
(Difference between revisions)
(New page: {{BNBK}} ==21 October 2008== *Optical Density S105 gene Restriction Digest with EcoRI and Spe I: 116.4 ng/ul *Optical Density S105 cassette after PCR Purification: 108.6 ng/ul *Optical D...) |
(→Restriction Digest of S105 cassette and PVJ4 cassette with EcoRI and SpeI:) |
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===Restriction Digest of S105 cassette and PVJ4 cassette with EcoRI and SpeI:=== | ===Restriction Digest of S105 cassette and PVJ4 cassette with EcoRI and SpeI:=== | ||
*S105 | *S105 | ||
| - | + | # 37.5 ul Milli-Q Water | |
| - | + | # 5 ul Multicore | |
| - | + | # .5 ul BSA | |
| - | + | # 5 ul DNA | |
| - | + | # 1 ul EcoRI and 1 ul Spe I | |
*PVJ4 | *PVJ4 | ||
| - | + | # 30.5 ul Milli-Q Water | |
| - | + | # 5 ul Multicore | |
| - | + | # .5 ul BSA | |
| - | + | # 12 ul DNA | |
| - | + | # 1 ul EcoRI and 1 ul Spe I | |
*50 ul total reaction volume | *50 ul total reaction volume | ||
Revision as of 14:39, 21 October 2008
21 October 2008
Restriction Digest of S105 cassette and PVJ4 cassette with EcoRI and SpeI:
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