Alberta NINT/13 June 2008

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(Difference between revisions)
Line 4: Line 4:
             I732018 was sequenced.
             I732018 was sequenced.
             I732018 was digested with XbaI + PstI.
             I732018 was digested with XbaI + PstI.
 +
 +
lab work (JD):
 +
            Quantitated K102005/BamH1 and Nco1
 +
            Ligated K102005/BamH1 and Nco1 with TA0/BamH1 and Nco1 (1/100 dilution)
 +
            Transformed XL1-B cells with ligation and negative control
 +
            Plated cells and left them for O/N

Revision as of 17:34, 13 June 2008

lab work (SD): 

            Using the transformation completed by Wayne (12/06/07) of XL1-B cells with I732018 and the overnight
            cultures, DNA was isolated by QIAprep standard miniprep protocol.
            I732018 was sequenced.
            I732018 was digested with XbaI + PstI.

lab work (JD): 

            Quantitated K102005/BamH1 and Nco1
            Ligated K102005/BamH1 and Nco1 with TA0/BamH1 and Nco1 (1/100 dilution)
            Transformed XL1-B cells with ligation and negative control
            Plated cells and left them for O/N
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