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Team:Hawaii/Notebook/2008-10-14
From 2008.igem.org
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(→Construction of secretion device (cont.)) |
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== Wetlab work == | == Wetlab work == | ||
===Construction of secretion device (cont.)=== | ===Construction of secretion device (cont.)=== | ||
| - | + | ||
[[Image:101408REdigest.png|right|thumb|300px|EtBr stained 2% agarose gel ran at 60V for 2 hours. Thirty microliters of RE digested DNA were loaded into each well.]] | [[Image:101408REdigest.png|right|thumb|300px|EtBr stained 2% agarose gel ran at 60V for 2 hours. Thirty microliters of RE digested DNA were loaded into each well.]] | ||
| + | :<strong> Grace</strong> | ||
:* Ran RE digests on gel | :* Ran RE digests on gel | ||
:* Extracted rgt1 and prpgt5 from gel | :* Extracted rgt1 and prpgt5 from gel | ||
Revision as of 07:42, 22 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of secretion device (cont.)
- Grace
- Ran RE digests on gel
- Extracted rgt1 and prpgt5 from gel
- Ligated:
- prpgt5 + BBpRL1383a-1
- nir + rgt1 + BBpRL1383a-1
- plac + rgt1 + BBpRL1383a-1
- nrsg6 (from 10/11) + BBpRL1383a-1
- J33207 + pRL1383a
Drylab Work
Sequencing analysis
- Grace
- prpgt, nrsg, sgt, and pgt sequences all returned inserts of E. coli genomic DNA instead of desired construct
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
