Revision as of 23:16, 14 June 2008

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Things we did today

Wetlab Work

Cell density test for PCC6803

Krystle

Plated 10^-5, 10^-7, 10^-7 dilutions of PCC6803 (OD750 = .071 and 1.15) on BG-11
Plates in SC incubator

Glycerol stocked Biobrick colonies

Krystle

Combined 0.4ml 80% glycerol w/ 0.8ml of transformed E. coli grown overnight in LB + antibiotic
Cryostock could not be made for pUC18 because of contamination in overnight culture

Made competent cells

Grace

Same protocol as 6/2 competent cells
OD600 = 0.4567 (overnight culture)
OD600 = 1.1-1.2 (after CCMB80 added)

Triparental conjugation

Grace

Streaked BG-11+sp+sm plates with conjugates
Incubated in light & CO₂ @ 30C in SC incubator

Discussion

For competent cells, should cool eppendorf tray at 4C. After aliquot, transfer tubes to -80C EtOH tray and freeze at -80C. GP suggests we aliquot with tubes in dry ice.

Was Callahan lab hood working?

Quote of the Day

"Yeah, I'm trying to stave off the real world by staying in school a little longer." - KS

Ooooooooooooooooooooooooh -KS, Yeah Krystle, it's shiny. -GK

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 36: / Line 36: @@
 = Quote of the Day =
 <blockquote>''"Yeah, I'm trying to stave off the real world by staying in school a little longer." - KS''</blockquote>
+<blockquote>''Ooooooooooooooooooooooooh'' -KS, ''Yeah Krystle, it's shiny.'' -GK</blockquote>
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Team:Hawaii/Notebook/2008-06-14

From 2008.igem.org