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Team:Hawaii/Notebook/2008-10-23
From 2008.igem.org
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(→Verification of transformants) |
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:* No change from yesterday | :* No change from yesterday | ||
:* Colony PCR of 6 colonies from sp<sub>40, 50, 75</sub> and 8 colonies from sp<sub>100</sub> plates | :* Colony PCR of 6 colonies from sp<sub>40, 50, 75</sub> and 8 colonies from sp<sub>100</sub> plates | ||
| + | :* Restreaked sp<sub>2.5</sub> blue colony, sp<sub>100</sub> #1 & 2 on LB+amp<sub>100</sub>, LB, LB+sp<sub>100</sub> + X-gal | ||
| + | |||
| + | ===Large scale plasmid prep=== | ||
| + | :<strong>Grace</strong> | ||
| + | |||
| + | :*From sp<sub>100</sub> plate: #1, 2 | ||
| + | :*From 9/28 sp<sub>2.5</sub> plate: #1 | ||
===Verification of transformants=== | ===Verification of transformants=== | ||
Revision as of 05:02, 24 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Antibiotic test
- Grace
- No change from yesterday
- Colony PCR of 6 colonies from sp40, 50, 75 and 8 colonies from sp100 plates
- Restreaked sp2.5 blue colony, sp100 #1 & 2 on LB+amp100, LB, LB+sp100 + X-gal
Large scale plasmid prep
- Grace
- From sp100 plate: #1, 2
- From 9/28 sp2.5 plate: #1
Verification of transformants
- Grace
| Plasmid | Colonies |
|---|---|
| J04430 | 0 |
| (-) on amp100 | 0 |
| BB-1 | 4 |
| (-) on sp100 | 0 |
- Colony PCR of 4 BB-1 transformants
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
