Team:Chiba/Calendar-Home/18 August 2008

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*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2007)
*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2007)
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''Transformation''
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'''[[Team:Chiba/protocol/transformation|Transformation]]'''
*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2006)
*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2006)
*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2007)
*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2007)

Revision as of 08:28, 24 October 2008

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17 August 2008 <|> 19 August 2008

Contents

Laboratory work

Team:Input

パーツ起こし

  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2006)
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2007)

Transformation

  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2006)
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2007)

competent cells : XL10Gold(Cm),LB-agar(KanR)

Team:communication

PCR
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070(2008)]
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076(2008)]
  • [http://partsregistry.org/Part:BBa_C0077 BBa_C0077(2008)]
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078(2008)]
  • [http://partsregistry.org/Part:BBa_C0079 BBa_C0079(2008)]
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620(2007)]･･･control


DNA Template 1
dNTP mix 10
Foward Primer 5
Reverse Primer 5
DNA polymerase 1
Thermopol Buffer 10
dH2O 68
TOTAL 100μL


95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃


--->Gel Check


Chiba-0818.JPG
Sample DNA 3
Loading Dye 2
dH2O 7
TOTAL 12μL
From left;
  • BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070] ->None
  • BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] ->None
  • BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] ->None
  • BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] ->None
  • BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079] ->None
  • BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control ->OK



Transformation

competent cells : XL10G

  • BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
  • BBa_C0076(2006) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates(19/8)
  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
  • BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
  • BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
  • BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
  • BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]


--->(20/8)Mini prep


Team:Output

no work

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