Team:Warsaw/Calendar-Main/27 September 2008

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<p><ol><li>Isolation of plasmid DNA from overnight cultures (it was really tough work...).</li>
<p><ol><li>Isolation of plasmid DNA from overnight cultures (it was really tough work...).</li>
-
<li>Control digestion of isolated plasmids with KpnI and BamHI (if mutagenesis really occured, ~550bp band should be visible).</li>
+
<li>Control digest of isolated plasmids with KpnI and BamHI (if mutagenesis really occured, ~550bp band should be visible).</li>
-
<li>Results of digestion:</li>
+
<li>Results of digest:</li>
<ul>
<ul>
<li>ADelL+KpnI and ADelP primers mutagenesis:</li>
<li>ADelL+KpnI and ADelP primers mutagenesis:</li>
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</ul>
</ul>
</ul>  
</ul>  
-
<li>Plasmids which gave positive digestion results prepared for sequencing (one sample from each variant which gave positive result).</li>
+
<li>Plasmids which gave positive digest results prepared for sequencing (one sample from each variant which gave positive result).</li>
</ol></p>
</ol></p>

Revision as of 12:45, 24 October 2008

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Mutagenesis of protein A

Paweł

  1. Isolation of plasmid DNA from overnight cultures (it was really tough work...).
  2. Control digest of isolated plasmids with KpnI and BamHI (if mutagenesis really occured, ~550bp band should be visible).
  3. Results of digest:
  4. Plasmids which gave positive digest results prepared for sequencing (one sample from each variant which gave positive result).

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