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Team:Chiba/Calendar-Home/31 August 2008
From 2008.igem.org
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*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]① | *[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]① | ||
| - | * | + | *[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]② |
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
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<tr> | <tr> | ||
<td>TOTAL(μL)</td> | <td>TOTAL(μL)</td> | ||
| - | <td> | + | <td>50μl</td><td>50μl</td> |
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 15:33, 24 October 2008
30 August 2008 <|> 1 September 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- Transformation
- Competent Cells : XL10Gold
- [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](Plac+RBS+LasI)
- [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](Plac+RBS+RhlI)
- ---> We saved these plates with a refrigerator.
- Digestion
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
- [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
Sample No. 1~3 4 Sample DNA(μL) 12 10 PstⅠ(μL) 0.2 0.2 XbaⅠ(μL) 0.2 - SpeⅠ(μL) - 0.4 Buffer 2(μL) - 1.5 Buffer 3(μL) 2 - BSA(μL) 2 1.5 dH2O(μL) 3.6 1.4 TOTAL(μL) 20 15
- --->(1/9)Gel Check
- (30/8)--->Mini prep
- --->Digestion test
- [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
Sample Single Digesiton 2~5 Double Digestion 2~5 Single Digestion T9002① Single Digesiton T9002② Double Digestion T9002①② Sample DNA(μL) 1 3 3 1 3 XbaⅠ(μL) 0.1 0.1 0.1 0.1 0.1 SpeⅠ(μL) - 0.1 - - 0.1 Buffer 2(μL) 0.9 0.8 0.9 0.9 0.8 BSA(μL) 1 1 1 1 1 dH2O(μL) 7 5 5 7 5 TOTAL(μL) 10 10 10 10 10
- --->Gel Check
Sample No. [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①② Single & Double Digestion Sample DNA(μL) 1 10 Loading Dye(μL) 1 2 dH2O(μL) 4 - TOTAL(μL) 6 12 - From Left
- Single Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
- Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
- Double Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
- Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
- From Left
Team:Output
- [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]①
- [http://partsregistry.org/Part:BBa_J52008 BBa_J52008]②
Sample No. ① ② DNA(μL) 10 10 SpeⅠ(μL) 1 0 PstⅠ(μL) 1 1 XbaⅠ(μL) 0 1 BSA(x100)(μL) 0.5 0.5 Buffer2(μL) 5 0 Buffer3(μL) 0 5 dH2O(μL) 28.5 28.5 TOTAL(μL) 50μl 50μl -->left for 3 hours at 37 degrees.
-->①20μl refine
-->②20μl refine
