Team:Chiba/Calendar-Home/31 August 2008

From 2008.igem.org

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</tr>
</tr>
<tr>
<tr>
-
<td>DNA(μL)</td>
+
<td>DNA</td>
<td>10</td><td>10</td>
<td>10</td><td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SpeⅠ(μL)</td>
+
<td>SpeⅠ</td>
<td>1</td><td>0</td>
<td>1</td><td>0</td>
</tr>
</tr>
<tr>
<tr>
-
<td>PstⅠ(μL)</td>
+
<td>PstⅠ</td>
<td>1</td><td>1</td>
<td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>XbaⅠ(μL)</td>
+
<td>XbaⅠ</td>
<td>0</td><td>1</td>
<td>0</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BSA(x100)(μL)</td>
+
<td>BSA(x100)</td>
<td>0.5</td><td>0.5</td>
<td>0.5</td><td>0.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer2(μL)</td>
+
<td>Buffer2</td>
<td>5</td><td>0</td>
<td>5</td><td>0</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer3(μL)</td>
+
<td>Buffer3</td>
<td>0</td><td>5</td>
<td>0</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O(μL)</td>
+
<td>dH<sub>2</sub>O</td>
<td>28.5</td><td>28.5</td>
<td>28.5</td><td>28.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL(μL)</td>
+
<td>TOTAL</td>
<td>50μl</td><td>50μl</td>
<td>50μl</td><td>50μl</td>
</tr>
</tr>

Revision as of 15:35, 24 October 2008

>Home | Notebook

30 August 2008 <|> 1 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

Transformation
Competent Cells : XL10Gold
  • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](Plac+RBS+LasI)
  • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](Plac+RBS+RhlI)
---> We saved these plates with a refrigerator.


Digestion
  1. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  2. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
  3. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  4. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
Sample No. 1~34
Sample DNA(μL)1210
PstⅠ(μL) 0.20.2
XbaⅠ(μL)0.2-
SpeⅠ(μL)-0.4
Buffer 2(μL) -1.5
Buffer 3(μL) 2-
BSA(μL) 21.5
dH2O(μL) 3.61.4
TOTAL(μL) 2015


--->(1/9)Gel Check


(30/8)--->Mini prep


--->Digestion test
  • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
Sample Single Digesiton 2~5Double Digestion 2~5Single Digestion T9002①Single Digesiton T9002②Double Digestion T9002①②
Sample DNA(μL)13313
XbaⅠ(μL)0.10.10.10.10.1
SpeⅠ(μL)-0.1--0.1
Buffer 2(μL) 0.90.80.90.90.8
BSA(μL) 11111
dH2O(μL) 75575
TOTAL(μL) 1010101010


--->Gel Check
Chiba-0831-p87.JPG
Sample No. [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①② Single & Double Digestion
Sample DNA(μL) 110
Loading Dye(μL) 12
dH2O(μL) 4-
TOTAL(μL) 612
From Left
  • Single Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
  • Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
  • Double Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
  • Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②



Team:Output

Digestion

  • [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]①
  • [http://partsregistry.org/Part:BBa_J52008 BBa_J52008]②
Sample No. ①②
DNA 1010
SpeⅠ 10
PstⅠ 11
XbaⅠ 01
BSA(x100) 0.50.5
Buffer2 50
Buffer3 05
dH2O 28.528.5
TOTAL 50μl50μl

-->left for 3 hours at 37 degrees.

-->①20μl refine

-->②20μl refine

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