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Team:Warsaw/Calendar-Main/1 September 2008
From 2008.igem.org
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<p><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A_alpha</a> in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain inoculated in liquid LB with chloramphenicol and ampicillin. | <p><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A_alpha</a> in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain inoculated in liquid LB with chloramphenicol and ampicillin. | ||
| + | </p> | ||
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| + | <h3>Blue-white screening and rifampicin test in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#gm">GM2163</a> - results</h3> | ||
| + | <h4>Michał L., Ewa</h4> | ||
| + | <p> | ||
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| + | <p>Blue colonies of Top10 strains transformed with pZC320 from various GM2163 strains were counted.<br> | ||
| + | Results (% of blue colonies):<br> | ||
| + | |||
| + | <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPMT5-omega</a> 100%<br> | ||
| + | |||
| + | <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5-AID</a> 100%<br> | ||
| + | |||
| + | <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5-AIDT7</a> 50%<br> | ||
| + | |||
| + | <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a> 0% (weird)<br> | ||
| + | </p> | ||
| + | <p> | ||
| + | <table id="result"> | ||
| + | <thead><b> Results of rifampicin test</b><br></thead> | ||
| + | <tr><th>Strain</th><th>Inducer / repressor</th><th>Number of colonies on rifampicin</th></tr> | ||
| + | <tr><th>GM2163</th><td>none</td><td>1</td></tr> | ||
| + | <tr><th>GM2163 pMPM-AID</th><td>0.1% Arabinose</td><td>47</td></tr> | ||
| + | <tr><th>GM2163 pMPM-AID</th><td>none</td><td>4</td></tr> | ||
| + | <tr><th>GM2163 pMPM-AID+T7</th><td>0.1% Arabinose</td><td>99</td></tr> | ||
| + | <tr><th>GM2163 pMPM-AID+T7</th><td>none</td><td>2</td></tr> | ||
| + | <tr><th>GM2163 pMPM-AIDT7</th><td>0.1% Arabinose</td><td>12</td></tr> | ||
| + | <tr><th>GM2163 pMPM-AIDT7</th><td>none</td><td>9</td></tr> | ||
| + | </table> | ||
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</p> | </p> | ||
</html> | </html> | ||
Revision as of 16:48, 24 October 2008
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Purification of proteins: A-alphaPiotrpET15b+A_alpha in Rosetta strain inoculated in liquid LB with chloramphenicol and ampicillin. Blue-white screening and rifampicin test in GM2163 - resultsMichał L., Ewa
Blue colonies of Top10 strains transformed with pZC320 from various GM2163 strains were counted.
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