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Team:Hawaii/Notebook/2008-10-24
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Prep for sequencing BB1=== :<strong> Grace</strong> :* ExoSAP'd BB1 PCr product and sent to CORE Hawaii for sequencing...) |
(→Antibiotic test) |
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| align="center"|sp<sub>100</sub> #2|| align="center"|No|| align="center"|No | | align="center"|sp<sub>100</sub> #2|| align="center"|No|| align="center"|No | ||
|- | |- | ||
| - | | rowspan="3" align="center"| | + | | rowspan="3" align="center"|LB||align="center"|sp<sub>2.5</sub> blue || align="center"|Yes|| align="center"|Yes, ~20 |
|- | |- | ||
| align="center"|sp<sub>100</sub> #1|| align="center"|Yes|| align="center"|No | | align="center"|sp<sub>100</sub> #1|| align="center"|Yes|| align="center"|No | ||
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:* Summary of task and what was done. Link to experiment for detailed notes if necessary. | :* Summary of task and what was done. Link to experiment for detailed notes if necessary. | ||
:* e.g. read through papers, worked on proposal, etc. | :* e.g. read through papers, worked on proposal, etc. | ||
| - | |||
= Discussion = | = Discussion = | ||
Revision as of 22:49, 24 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Prep for sequencing BB1
- Grace
- ExoSAP'd BB1 PCr product and sent to CORE Hawaii for sequencing
Plasmid prep of parts to send to iGEM
- Grace
| Plasmid | Concentration |
|---|---|
| nir | |
| slr | |
| pilA | |
| GFPf | |
| nir+rbs | |
| slr+GFPf | |
| BBpRL1383a #1 from sp100 plate |
Antibiotic test
- Grace
| Plate | Colony | Growth? | Blue? |
|---|---|---|---|
| LB+amp100 | sp2.5 blue | No | No |
| sp100 #1 | No | No | |
| sp100 #2 | No | No | |
| LB | sp2.5 blue | Yes | Yes, ~20 |
| sp100 #1 | Yes | No | |
| sp100 #2 | Yes | No | |
| LB+sp100 | sp2.5 blue | No | No |
| sp100 #1 | Yes | No | |
| sp100 #2 | Yes | No |
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
