Team:NTU-Singapore/Notebook/13 June 2008

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*Chin Chong
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=Friday 13 June=
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===Chin Chong===
**BL21 cells, containing the LacI-GFP, that were inoculated on thursday were not growing well as they were placed too long in the incubator
**BL21 cells, containing the LacI-GFP, that were inoculated on thursday were not growing well as they were placed too long in the incubator
**Regrew/Inoculate the BL21 cells at 5pm so that overnight growing of 16hrs would be acheived
**Regrew/Inoculate the BL21 cells at 5pm so that overnight growing of 16hrs would be acheived
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***Varified the correct usage from the student, Zhang Zhengwen, who adviced on some slight modifications to the protocol set for the characterization experiment
***Varified the correct usage from the student, Zhang Zhengwen, who adviced on some slight modifications to the protocol set for the characterization experiment
**Received the primer designs from 1st Base for: supD, T7ptag and direction checks for T7ptag as well as that for LsrA
**Received the primer designs from 1st Base for: supD, T7ptag and direction checks for T7ptag as well as that for LsrA
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Revision as of 04:39, 25 October 2008

Friday 13 June

Chin Chong

    • BL21 cells, containing the LacI-GFP, that were inoculated on thursday were not growing well as they were placed too long in the incubator
    • Regrew/Inoculate the BL21 cells at 5pm so that overnight growing of 16hrs would be acheived
    • Prepared 20 plates of agar containing Chloranphenicol and 20 plates of agar containing Kanamycin
    • Approached Phd students from BIE whom had experience in using the multiplate Fluroescene and luminescene reader
      • Varified the correct usage from the student, Zhang Zhengwen, who adviced on some slight modifications to the protocol set for the characterization experiment
    • Received the primer designs from 1st Base for: supD, T7ptag and direction checks for T7ptag as well as that for LsrA
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