Team:Warsaw/Calendar-Main/24 May 2008

From 2008.igem.org

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<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5-AID</a></th><td>none</td><td>10</td></tr>
<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5-AID</a></th><td>none</td><td>10</td></tr>
<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5-AID</a></th><td>0.1% Arabinose</td><td>154</td></tr>
<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5-AID</a></th><td>0.1% Arabinose</td><td>154</td></tr>
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<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5-AID</a></th><td>none</td><td>11</td></tr>
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<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99a-AID</a></th><td>none</td><td>11</td></tr>
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<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5-AID</a></th><td>0.5 mM IPTG</td><td>59</td></tr>
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<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99a-AID</a></th><td>0.5 mM IPTG</td><td>59</td></tr>
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Revision as of 09:37, 25 October 2008

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Results of rifampicin test

Piotr

StrainInducer / repressorNumber of colonies on rifampicin
Top10none19
Top10 pMPM-T5-AIDnone10
Top10 pMPM-T5-AID0.1% Arabinose154
Top10 pTrc99a-AIDnone11
Top10 pTrc99a-AID0.5 mM IPTG59

Conclusion: AID seems to work!

Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

  1. Isolation of hypothetical pMPM-T5 with transcriptional fusion from cultures.
  2. Isolation of hypothetical pMPM-T5 with translational fusion from cultures.
  3. Control digest of pMPM-T5 with transcriptional fusionwith EcoRI (EcoRI buffer).
  4. Control digest of pMPM-T5 with translational fusion with BamHI (BamHI buffer).
  5. Gel electrophoresis (still no proper colonies found).

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