Team:Brown/Notebook/Protocols
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(New page: {{BrownTemp}} =Protocols= PROTOCOLS: ====Miniprep Prodedure==== *The purpose of this procedure is to extract plasmid DNA and separate it from chromosal DNA in a cell. All DNA (chromosal...) |
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*The purpose of this procedure is to extract plasmid DNA and separate it from chromosal DNA in a cell. All DNA (chromosal and plasmid) is denatured in the first step. Plasmid DNA, unlike chromosomal DNA, forms linked rings upon denaturation. Next, DNA is renatured with the use of buffers. Chromosomal DNA remains denatured because of the inefficiency of re-ligation of multiple pieces and strands of DNA. The plasmid DNA renatures and is thereby, separated from chromosomal DNA. | *The purpose of this procedure is to extract plasmid DNA and separate it from chromosal DNA in a cell. All DNA (chromosal and plasmid) is denatured in the first step. Plasmid DNA, unlike chromosomal DNA, forms linked rings upon denaturation. Next, DNA is renatured with the use of buffers. Chromosomal DNA remains denatured because of the inefficiency of re-ligation of multiple pieces and strands of DNA. The plasmid DNA renatures and is thereby, separated from chromosomal DNA. |
Revision as of 14:42, 25 October 2008
ProtocolsMiniprep Prodedure
Gel Electrophoresis
Ligation Reaction Procedure
Transformation Procedure
Ethanol Precipitation
Gel Extraction
The contents of the tube should be yellow after incubation.
Calf CIP - Calf Intestinal Alkaline Phosphatase
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