"
Team:Warsaw/Calendar-Main/10 September 2008
From 2008.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
<h4>Piotr, Emila</h4> | <h4>Piotr, Emila</h4> | ||
| - | <ol><li>Inoculation of all OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various cocnentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).</li> | + | <ol><li>Inoculation of all pACYC177+OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various cocnentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).</li> |
| - | <li><p>Inoculation of | + | <li><p>Inoculation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>OmpA_A_alpha</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega to LB + 0.25 mM IPTG and kanamycin.</li> |
<li>Measurement of growth after 6 hours.</li></ol> | <li>Measurement of growth after 6 hours.</li></ol> | ||
Revision as of 20:27, 25 October 2008
 |
|
||||||||
 |
|
||||||||
Tests for ampicillin resistance given by protein added to mediumPiotr, Emila
Bacterial growth depending on the amount of prey protein added to the medium. The amount is expressed in picomoles. Z_omega: 20323.75 Da, Z_alpha: 28734.24 Da, A_alpha: 37052.21 Da.
Z_omega was added to bacteria culture expressing A_alpha.
|
|
