Team:Warsaw/Calendar-Main/10 September 2008

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Tests for ampicillin resistance given by protein added to medium

Piotr, Emila

Inoculation of all pACYC177+OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various cocnentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).
Inoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_A_omega, pACYC177+OmpA_Z_omega, pACYC177+OmpA_alpha and pACYC177+OmpA_omega to LB + 0.25 mM IPTG and kanamycin.
Measurement of growth after 6 hours.

Prey: Z-omega	amount in picomoles per mL	4,920	12,301	24,602	61,504	123,009	246,018	369,026
	OD	0	0,21	0,34	0,62	0,89	0,91	0,92
Prey: Z-alfa	amount in picomoles per mL	4,333	8,692	17,383	43,485	86,996	173,991	260,987
	OD	0	0,25	0,42	0,71	0,9	0,95	0,98
Prey: A-alfa	amount in picomoles per mL	5,600	11,234	22,468	56,204	112,443	224,885	337,328
	OD	0	0,19	0,38	0,65	0,85	1,00	1,03

Bacterial growth depending on the amount of prey protein added to the medium. The amount is expressed in picomoles per mL of medium. Z_omega: 20323.75 Da, Z_alpha: 28734.24 Da, A_alpha: 37052.21 Da.

Z_omega was added to bacteria culture expressing pACYC177+OmpA_A_alpha
Z_alpha was added to bacteria culture expressing pACYC177+OmpA_A_omega.
A_alpha was added to bacteria culture expressing pACYC177+OmpA_Z_omega.

@@ Line 22: / Line 22: @@
 <tr><th>Prey: A-alfa</th><th>	amount in picomoles per mL</th><th>	5,600	</th><th>11,234</th><th>	22,468	</th><th>56,204</th><th>112,443</th><th>	224,885</th><th>	337,328</th></tr>
-<tr><th>	</th><th>	OD	</th><th>0	</th><th>0,19	</th><th>0,38	</th><th>0,65</th><th>	0,85	</th><th>1	</th><th>1,03</th></tr>
+<tr><th>	</th><th>	OD	</th><th>0	</th><th>0,19	</th><th>0,38	</th><th>0,65</th><th>	0,85	</th><th>1,00	</th><th>1,03</th></tr>
 </table>