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Team:Peking University
From 2008.igem.org
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<area shape="rect" coords="446,82,469,149" href="http://openwetware.org/wiki/User:Wenjun_Shao"> | <area shape="rect" coords="446,82,469,149" href="http://openwetware.org/wiki/User:Wenjun_Shao"> | ||
Revision as of 12:52, 26 October 2008
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | [http://www.openwetware.org/wiki/IGEM:Peking_University/2008 Our OWW] | [http://www.openwetware.org/wiki/IGEM:Peking_University/2008/Notebook Notebook] |
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A Genetic Circuit for Directed Evolution in vivo
Directed evolution is a powerful tool for answering scientific questions or constructing novel biological systems. Here we present a simple genetic circuit for in vivo directed evolution which comprises minimal elements for random mutation and artificial selection. We engineer yeast to generate the DNA mutator hAID, an essential protein in adaptive immunity, and target it specifically to a gene of interest. The target gene will be mutated and consequently promptly evolves. By linking the expression of hAID repressor LacI and favorite gene functionality, the mutation rate inversely correlates between the functionality of the desired gene and hAID. This circuit may be adopted for in vivo evolution in eukaryotic system on genetically encoded targets. It has a variety of potential applications in academic and industrial contexts, theoretically most inter-molecular interaction that involves proteins and RNAs.
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Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs) |  Your team picture |
| Team Example 2 |
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