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Team:Montreal/DNA Agarose Gel
From 2008.igem.org
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1. Measure 50mL of TAE buffer and mix with 0.5g of Agarose powder. | 1. Measure 50mL of TAE buffer and mix with 0.5g of Agarose powder. | ||
Revision as of 19:42, 17 June 2008
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Protocol
1. Measure 50mL of TAE buffer and mix with 0.5g of Agarose powder.
2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling).
3. Add 1.5µL of Ethidium Bromide and swirl until a fine mixture is seen.
4. Pour the mixture onto the casting tray (make sure that each end is taped), with a comb inserted in one of the ends.
5. Wait for the gel to solidify over time.
6. Once hardened, remove the comb and load the gel into the electrophoresis box.
