Team:Warsaw/Calendar-Main/16 July 2008

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Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin

We have massive outbreak of some virulent bacteriophage strain in our lab. All our current E. coli cultures are gone :-(. Until we get rid of the phage there will be no microbiological work in our lab. Sorry.

Cloning of protein Z DNA to OmpA constructs

Michał K.

Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_omega).
Control digest of isolated plasmids with BamHI and SacI (BamHI buffer).
Gel electrophoresis - both checked colones are good (Fig. 1.).

Fig. 1. Control SacI/BamHI digests of isolated plasmids 1. Marker 2-3. digested plasmids pACYC177+OmpA_Z_omega

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 <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a>). </li>
 <li> Control
-<a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer) (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_July_2008#fig1">Fig. 1.</a>).</li> <li> Gel electrophoresis - both checked colones are good .</li>
+<a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer).</li> <li> Gel electrophoresis - both checked colones are good (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_July_2008#fig1">Fig. 1.</a>).</li>
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