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Team:Heidelberg/Notebook/Sensing Group/Notebook/11thweek
From 2008.igem.org
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== Monday, 10/13/2008 == | == Monday, 10/13/2008 == | ||
| + | |||
| + | * Miniprep of LuxS_mut_EcoRI and F1-YFP, F2-YFP | ||
| + | * Digestion of Fusion-YFP with BamHI (NEBuffer3 + BSA) | ||
| + | ** Insert: 5937 bp and 1998 bp | ||
| + | ** No Insert: 7202 bp | ||
| + | * Digestion of LuxS with EcoRI/NdeI (NEBuffer EcoRI) | ||
| + | ** Insert: 2773 bp and 1862 bp | ||
| + | ** 4635 bp | ||
| + | * Fusion-PCR for LuxS standardization with Phusion | ||
| + | ** 30s @ 98°C || 10s @ 98°C | 30s @ 55°C | 1min @ 72°C || 5min @ 72°C | 4°C (30 cycles) | ||
== Tuesday, 10/14/2008 == | == Tuesday, 10/14/2008 == | ||
Revision as of 19:43, 26 October 2008
Contents |
Monday, 10/13/2008
- Miniprep of LuxS_mut_EcoRI and F1-YFP, F2-YFP
- Digestion of Fusion-YFP with BamHI (NEBuffer3 + BSA)
- Insert: 5937 bp and 1998 bp
- No Insert: 7202 bp
- Digestion of LuxS with EcoRI/NdeI (NEBuffer EcoRI)
- Insert: 2773 bp and 1862 bp
- 4635 bp
- Fusion-PCR for LuxS standardization with Phusion
- 30s @ 98°C || 10s @ 98°C | 30s @ 55°C | 1min @ 72°C || 5min @ 72°C | 4°C (30 cycles)
