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Team:Heidelberg/Notebook/Sensing Group/Notebook/11thweek
From 2008.igem.org
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== Tuesday, 10/14/2008 == | == Tuesday, 10/14/2008 == | ||
| + | * PCR Purification, Digestion of LuxS and pSB2K3 with EcoRI/PstI (EcoRI buffer + BSA). 1h @ 37°C | ||
| + | * Fusion PCR for LuxS with Phusion and Annealing Temperature Gradient (50-60°C) | ||
| + | [[Image:HD 081014-LuxS FusionPCR.png|center|thumb|300px|Fused LuxS parts for standardization seem positive]] | ||
| + | * Transformation of Fusion-YFP into MG1655 | ||
== Wednesday, 10/15/2008 == | == Wednesday, 10/15/2008 == | ||
Revision as of 19:45, 26 October 2008
Contents |
Monday, 10/13/2008
- Miniprep of LuxS_mut_EcoRI and F1-YFP, F2-YFP
- Digestion of Fusion-YFP with BamHI (NEBuffer3 + BSA)
- Insert: 5937 bp and 1998 bp
- No Insert: 7202 bp
- Digestion of LuxS with EcoRI/NdeI (NEBuffer EcoRI)
- Insert: 2773 bp and 1862 bp
- 4635 bp
- Fusion-PCR for LuxS standardization with Phusion
- 30s @ 98°C || 10s @ 98°C | 30s @ 55°C | 1min @ 72°C || 5min @ 72°C | 4°C (30 cycles)
Tuesday, 10/14/2008
- PCR Purification, Digestion of LuxS and pSB2K3 with EcoRI/PstI (EcoRI buffer + BSA). 1h @ 37°C
- Fusion PCR for LuxS with Phusion and Annealing Temperature Gradient (50-60°C)
- Transformation of Fusion-YFP into MG1655
