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Team:Warsaw/Calendar-Main/1 September 2008
From 2008.igem.org
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<h3>Purification of proteins: A-alpha</h3><h4>Piotr</h4> | <h3>Purification of proteins: A-alpha</h3><h4>Piotr</h4> | ||
| - | <p><a href=https://2008.igem.org/Wiki/Team:Warsaw/ | + | <p>Liquid LB with chloramphenicol and ampicillin was inoculated with <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A_alpha</a>. |
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Purification of proteins: A-alphaPiotrLiquid LB with chloramphenicol and ampicillin was inoculated with Rosetta strain carrying pET15b+A_alpha. Blue-white screening and rifampicin test in GM2163Michał L., Ewa
Blue colonies of Top10 strains transformed with pZC320 from various GM2163 strains were counted.
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