Team:Hawaii/Antibiotic test for aadA construct

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::*constructs
::*constructs
::*(+) control pSMC121 (contains ''aadA'' gene)
::*(+) control pSMC121 (contains ''aadA'' gene)
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::*(-) control pSB1A3  
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::*(-) control pSB1A3
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:*<strong>Transformation</strong> of the plasmid from one of these constructs into ''E. coli'' and plate on LB+Agar with varying concentrations of SmSp.
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::* DH5-a
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::* DB3.1
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::* controls: (+) pSMC121, (-) no plasmid
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== Results ==
== Results ==

Revision as of 02:04, 27 October 2008

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Contents

Antibiotic test for aadA construct

  • The gene for Sp and Sm resistance was constructed in BioBrick format. It was amplified from pRL1383a with primers adding BioBrick ends, and the lac promoter and rbs were ligated to it.
  • This construct was tested for antibiotic resistance.

Methods

  • Sequencing confirmation
  • Streak on LB+Agar plates with Sm50Sp10
  • constructs
  • (+) control pSMC121 (contains aadA gene)
  • (-) control pSB1A3

Results

  • Sequencing confirmation
  • Streak
    The plate streaked with multiple aadA constructs + controls.
  • All four of the constructs grew and the positive control grew while the negative control did not grow.


Discussion

  • What was learned and how to do future experiments differently.


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