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Team:Valencia/Notebook/October
From 2008.igem.org
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=== October 2nd === | === October 2nd === | ||
- Team meeting: We talked about the biobricks and some experiments left. | - Team meeting: We talked about the biobricks and some experiments left. | ||
| + | |||
| + | === October 7th === | ||
| + | - We repeated the previous experiment:<br> | ||
| + | Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
| + | 1 % Galactose induction at the beginning. Initial O.D. 2 <br> | ||
| + | 270 rpm. <br> | ||
| + | Initial temperature 28 ºC.<br> | ||
| + | |||
| + | === October 13th === | ||
| + | - genomic DNA extraction of our strains.<br> | ||
| + | - we carried PCR. | ||
| + | |||
| + | === October 14th === | ||
| + | - Agarose gel for check our PCR | ||
=== October 17th === | === October 17th === | ||
Revision as of 12:25, 27 October 2008
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October 1st
- We repeated the previous experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 2
270 rpm.
Initial temperature 28 ºC.
October 2nd
- Team meeting: We talked about the biobricks and some experiments left.
October 7th
- We repeated the previous experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 2
270 rpm.
Initial temperature 28 ºC.
October 13th
- genomic DNA extraction of our strains.
- we carried PCR.
October 14th
- Agarose gel for check our PCR
October 17th
- Team meeting: We discussed several things that need to be finished, such us sending DNA, presentation, poster, wiki...
