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USTC/Notebook/PCR&Colony PCR
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(New page: Colony PCR 2 uL primer #1 (to 10uM) 2 uL primer #2 0.4 uL DNTPs 2 uL Buffer 5 uL colony culture 0.2 uL Taq 10.4 uL H20 92C 1:30 (92 0:30 50 :30 72 1:30) repeat x30 72 10:00) |
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Revision as of 13:26, 27 October 2008
Colony PCR
2 uL primer #1 (to 10uM) 2 uL primer #2 0.4 uL DNTPs 2 uL Buffer 5 uL colony culture 0.2 uL Taq 10.4 uL H20
92C 1:30 (92 0:30 50 :30 72 1:30) repeat x30 72 10:00
