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Alberta NINT/17 June 2008
From 2008.igem.org
(Difference between revisions)
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| + | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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lab work (SD): | lab work (SD): | ||
Transformed XL1-B cells with: | Transformed XL1-B cells with: | ||
| Line 8: | Line 10: | ||
Re-created K102003 with new LacZ parts I732018.1 and I732018.2 | Re-created K102003 with new LacZ parts I732018.1 and I732018.2 | ||
Ligated K102001 with LacZ Parts | Ligated K102001 with LacZ Parts | ||
| - | Transformed and plated XL1-B cells with K102003(1) (containing LacZ part I732018.1) and K102003(2) (containing LacZ part I732018.2) | + | Transformed and plated XL1-B cells with K102003(1) (containing LacZ part I732018.1) and K102003 |
| - | Our plates of K102007 from yesterday with 1/10 1/100 and 1/1000 dilutions didn't seem to make any difference so we are going to recut TA0 | + | (2) (containing LacZ part I732018.2) |
| + | Our plates of K102007 from yesterday with 1/10 1/100 and 1/1000 dilutions didn't seem to make any | ||
| + | difference so we are going to recut TA0 | ||
Restriction digest of TA0 with BamH1 and Nco1 | Restriction digest of TA0 with BamH1 and Nco1 | ||
Nucleotide removal of TA0/B+N | Nucleotide removal of TA0/B+N | ||
Created glycerol stock of K102005 | Created glycerol stock of K102005 | ||
Made fresh O/N of K102005 to be used tomorrow | Made fresh O/N of K102005 to be used tomorrow | ||
Revision as of 20:25, 19 June 2008
lab work (SD):
Transformed XL1-B cells with:
K102000 + I732018.1
K102000 + I732018.2
K102000 (negative control)
lab work (JD):
Re-created K102003 with new LacZ parts I732018.1 and I732018.2
Ligated K102001 with LacZ Parts
Transformed and plated XL1-B cells with K102003(1) (containing LacZ part I732018.1) and K102003
(2) (containing LacZ part I732018.2)
Our plates of K102007 from yesterday with 1/10 1/100 and 1/1000 dilutions didn't seem to make any
difference so we are going to recut TA0
Restriction digest of TA0 with BamH1 and Nco1
Nucleotide removal of TA0/B+N
Created glycerol stock of K102005
Made fresh O/N of K102005 to be used tomorrow
