"
Alberta NINT/12 June 2008
From 2008.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| + | |||
| + | [[Alberta_NINT/11_June_2008 | < Previous entry]] | ||
lab work (SD): | lab work (SD): | ||
| Line 17: | Line 19: | ||
Reviewing the parts registry, it was noted that the wrong plate well was used to obtain I732018. | Reviewing the parts registry, it was noted that the wrong plate well was used to obtain I732018. | ||
I732018 was re-isolated from the correct well, transformed into XL1-B and made into overnights. | I732018 was re-isolated from the correct well, transformed into XL1-B and made into overnights. | ||
| + | |||
| + | [[Alberta_NINT/13_June_2008 | Next entry >]] | ||
Revision as of 21:35, 19 June 2008
lab work (SD):
Isolated DNA from 11/06/08 overnights using QIAprep standard miniprep protocol.
Set up sequencing reaction for I732018.1 and I732018.2.
Digested BBa_I732018.1 and BBa_I732018.2 with XbaI + PstI. Run on 2% agarose gel (110 V, 50 min).
lab work (JD):
Mini-prep of LacZ part I732018
Digested LacZ with XbaI and PstI
Digested K102005 with BamH1 aqnd NcoI
Ran a gel, (see note below about LacZ part)
Gel purified my K102005 band
Gel electrophoresis revealed uncut plasmid, indicating that "I732018" was not the desired part.
Reviewing the parts registry, it was noted that the wrong plate well was used to obtain I732018.
I732018 was re-isolated from the correct well, transformed into XL1-B and made into overnights.
