Alberta NINT/20 June 2008

From 2008.igem.org

(Difference between revisions)
Sdeane (Talk | contribs)
(New page: lab work (SD): Digested K102002.1-.6 and freshly annealed TA0 with BamHI + NcoI. Incubated at 37 C for 2 hrs. Ran K102002.1-.6 on 2% agarose gel at 1...)
Newer edit →

Revision as of 17:20, 20 June 2008

lab work (SD): 

            Digested K102002.1-.6 and freshly annealed TA0 with BamHI + NcoI.
            Incubated at 37 C for 2 hrs.
            Ran K102002.1-.6 on 2% agarose gel at 110V for 50 min.
            Purified TA0 using QIAquick nucleotide removal protocol.
            Quantified DNA for K102002 and TA0.
            Ligated TA0 into K102002.1-.6 and incubated at 16 C for 30 min.
Retrieved from "http://2008.igem.org/Alberta_NINT/20_June_2008"