Team:BrownTwo/Protocols/compcells

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(New page: {{limitertemp}} =Making Ultracompetent Cells= *Transformation can be a major roadblock in assembling DNA constructs. Ligations can be hard to transform, especially with large inserts. D...)
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Making Ultracompetent Cells

  • Transformation can be a major roadblock in assembling DNA constructs. Ligations can be hard to transform, especially with large inserts. DNA blotted on paper by the registry tends to be even harder. We have had much better luck with the following protocol from Inoue than the standard CaCl2 method:
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