Team:BrownTwo/Limiter/altdesign

Alternative construction and design possiblities

• Smolke’s gal2 knockout scheme for using the GAL1 promoter
• Finding additional inducible/repressible promoters to use in the system
• Ribozyme switch inhibition to knockdown leakiness of Repressor#3 (this would need to designed so that the aptamer on the repressor #3 mRNA responds to Repressor #2 protein)
• alternatively, could consider having the promoter for construct Y produce an shRNA instead of a repressor protein
• need to consider the possibility for regulation on both levels of protein synthesis

Possible further modifications to these transcription factors include the use of different regulation domains,

Involves the consideration of multiple binding sites