Team:Michigan/9 October 2008
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==<B> Why can't E.coli metabolize hot dogs? I'm afraid they just can't "digest" them </B>== | ==<B> Why can't E.coli metabolize hot dogs? I'm afraid they just can't "digest" them </B>== | ||
- | *PCR clean-up (quenches digests) on our digests from yesterday | + | *[https://2008.igem.org/Team:Michigan/Notebook/PCRProtocol <u>PCR clean-up</u>] (quenches digests) on our digests from yesterday |
- | *Attempted two ligations: pCLOCK1,nifA (NdeI/SpeI) and BBa R0010 (lacI inducible lac promoter), nifA (R0010 with SpeI, nifA with XbaI/SpeI); transformed onto Ampicillin plates | + | *Attempted two [https://2008.igem.org/Team:Michigan/Notebook/LigationProtocol <u>ligations</u>]: pCLOCK1,nifA (NdeI/SpeI) and BBa R0010 (lacI inducible lac promoter), nifA (R0010 with SpeI, nifA with XbaI/SpeI); [https://2008.igem.org/Team:Michigan/Notebook/TransformationProtocol <u>transformed</u>] onto Ampicillin plates |
- | *Ran analytical gels on the digests too - all lengths matched up, except for our midi-prep of pCLOCK2; something messed up there, as the ladder in the lane next door didn't work either; need to re-check pCLOCK2 | + | *Ran [https://2008.igem.org/Team:Michigan/Notebook/GelProtocol <u>analytical gels</u>] on the digests too - all lengths matched up, except for our midi-prep of pCLOCK2; something messed up there, as the ladder in the lane next door didn't work either; need to re-check pCLOCK2 |
Latest revision as of 07:46, 28 October 2008
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October 9, 2008Why can't E.coli metabolize hot dogs? I'm afraid they just can't "digest" them
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