Team:BrownTwo/Limiter/altdesign

From 2008.igem.org

(Difference between revisions)
(New page: {{limitertemp}} Alternative construction and design possiblities *Smolke’s gal2 knockout scheme for using the GAL1 promoter *Finding additional inducible/repressible promoters to use in...)
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Alternative construction and design possiblities
Alternative construction and design possiblities
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*up-limiter
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*Smolke’s gal2 knockout scheme for using the GAL1 promoter
*Smolke’s gal2 knockout scheme for using the GAL1 promoter
*Finding additional inducible/repressible promoters to use in the system
*Finding additional inducible/repressible promoters to use in the system
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*Ribozyme switch inhibition to knockdown leakiness of Repressor#3 (this would need to designed so that the aptamer on the repressor #3 mRNA responds to Repressor #2 protein)
 
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*alternatively, could consider having the promoter for construct Y produce an shRNA instead of a repressor protein
 
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*need to consider the possibility for regulation on both levels of protein synthesis
 
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Possible further modifications to these transcription factors include the use of different regulation domains,
 
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Involves the consideration of multiple binding sites
 

Revision as of 07:47, 28 October 2008



Alternative construction and design possiblities

  • up-limiter
  • Smolke’s gal2 knockout scheme for using the GAL1 promoter
  • Finding additional inducible/repressible promoters to use in the system
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