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Team:Edinburgh/cex
From 2008.igem.org
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See below for mutagenic primers.<br /> | See below for mutagenic primers.<br /> | ||
| - | === BIOBRICK PRIMERS | + | === BIOBRICK<sup>TM</sup> PRIMERS === |
'''Forward:''' CGT t tctagATG CCT AGG ACC ACG CC<br /> | '''Forward:''' CGT t tctagATG CCT AGG ACC ACG CC<br /> | ||
Latest revision as of 10:51, 28 October 2008
Contents |
cex
For better alignment see .txt file
AMINO ACID SEQUENCE
MPRTTPAPGHPARGARTAlRTTLAAAAATLVVGATVvlPAQAATTLKEAA
DGAGRDFGFALDPNRLSEAQYKAIADSEFNLVVAENAMKWDATEPSQNSF
SFGAGDRVASYAADTGKElYGHTLVWHSQlPDWAKNLNGSAFESAMVNHV
TKVADHFEGKVASWDVVNEAFADGGGRRQDSAFQQKLGNGYIETAFRAAR
AADPTAKlCINDYNVEGINAKSNSLYDLVKDFKARGVPLDCVGFQSHLIV
GQvPGDFRQNlQRFADlGvDvRITELDIRMRTPSDATKLATQAADYKKVv
QACMQvTRCQGvTVWGITDKYSWvPDVFPGEGAAlvWDASYAKKPAYAAv
MEAFGASPTPTPTTPTPTPTTPTPTPTSGPAGCQvlWGVNQWNTGFTANV
TvKNTSSAPVDGWTLTFSFPSGQQVTQAWSSTVTQSGSAvTVRNAPWNGS
IPAGGTAQFGFNGSHTGTNAAPTAFSLNGTPCTVG*
DNA SEQUENCE
atgcctaggaccacgcccgcacccggccacccggcccgcggcgcccgcac 50
tacggatcctggtgcgggcgtgggccggtgggccgggcgccgcgggcgtg
cgctctgcgcacgacgctcgccgccgcggcggcgacgctcgtcgtcggcg 100
gcgagacgcgtgctgcgagcggcggcgccgccgctgcgagcagcagccgc
ccacggtcgtgctgcccgcccaggccgcgaccacgctcaaggaggccgcc 150
ggtgccagcacgacgggcgggtccggcgctggtgcgagttcctccggcgg
gacggcgccggccgggacttcggcttcgcgctcgaccccaaccggctctc 200
ctgccgcggccggccctgaagccgaagcgcgagctggggttggccgagag
ggaggcgcagtacaaggcgatcgccgacagcgagttcaacctcgtcgtcg 250
cctccgcgtcatgttccgctagcggctgtcgctcaagttggagcagcagc
ccgagaacgcgatgaagtgggacgccaccgagccctcgcagaacagcttc 300
ggctcttgcgctacttcaccctgcggtggctcgggagcgtcttgtcgaag
tccttcggcgcgggcgaccgcgtcgcgagctacgccgccgacaccggcaa 350
aggaagccgcgcccgctggcgcagcgctcgatgcggcggctgtggccgtt
ggagctgtacggccacacgctcgtatggcactcgcagctgcccgactggg 400
cctcgacatgccggtgtgcgagcataccgtgagcgtcgacgggctgaccc
cgaagaacctcaacggctccgcgttcgagagcgcgatggtcaaccacgtg 450
gcttcttggagttgccgaggcgcaagctctcgcgctaccagttggtgcac
acgaaggtcgccgaccacttcgagggcaaggtcgcgtcgtgggacgtcgt 500
tgcttccagcggctggtgaagctcccgttccagcgcagcaccctgcagca
caacgaggcgttcgccgacggcggcggccgccggcaggactcggcgttcc 550
gttgctccgcaagcggctgccgccgccggcggccgtcctgagccgcaagg
agcagaagctcggcaacggctacatcgagaccgcgttccgggcggcacgt 600
tcgtcttcgagccgttgccgatgtagctctggcgcaaggcccgccgtgca
gcggcggacccgaccgccaagctgtgcatcaacgactacaacgtcgaggg 650
cgccgcctgggctggcggttcgacacgtagttgctgatgttgcagctccc
catcaacgcgaagagcaactcgctctacgacctcgtcaaggacttcaagg 700
gtagttgcgcttctcgttgagcgagatgctggagcagttcctgaagttcc
cgcgcggcgtcccgctcgactgcgtcgggttccagtcgcacctcatcgtc 750
gcgcgccgcagggcgagctgacgcagcccaaggtcagcgtggagtagcag
ggccaggtgccgggcgacttccggcagaacctgcagcggttcgcggacct 800
ccggtccacggcccgctgaaggccgtcttggacgtcgccaagcgcctgga
gggcgtggacgtgcgcatcaccgagctcgacatccgcatgcggacgccct 850
cccgcacctgcacgcgtagtggctcgagctgtaggcgtacgcctgcggga
ccgacgcgaccaagctcgcgacccaggcggccgactacaagaaggtcgtg 900
ggctgcgctggttcgagcgctgggtccgccggctgatgttcttccagcac
caggcctgcatgcaggtgacccgctgccagggcgtgaccgtctggggcat 950
gtccggacgtacgtccactgggcgacggtcccgcactggcagaccccgta
caccgacaagtactcgtgggtgccggacgtcttcccgggcgagggggccg 1000
gtggctgttcatgagcacccacggcctgcagaagggcccgctcccccggc
cgctggtgtgggacgcgagctacgccaagaagccggcctacgccgccgtg 1050
gcgaccacaccctgcgctcgatgcggttcttcggccggatgcggcggcac
atggaggccttcggcgcgagcccgacgccgacgcccaccacgccgacccc 1100
tacctccggaagccgcgctcgggctgcggctgcgggtggtgcggctgggg
gacgcccacgacgccgacgccgaccccgacgtccggtccggccgggtgcc 1150
ctgcgggtgctgcggctgcggctggggctgcaggccaggccggcccacgg
aggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtc 1200
tccacgacaccccgcagttggtcaccttgtggccgaagtggcgcttgcag
accgtgaagaacacgtcctccgctccggtcgacggctggacgctcacgtt 1250
tggcacttcttgtgcaggaggcgaggccagctgccgacctgcgagtgcaa
cagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtca 1300
gtcgaagggcaggccggtcgtccagtgggtccgcacctcgagctgccagt
cgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcg 1350
gcgtcaggccgagccggcactgccaggcgttgcggggcaccttgccgagc
atcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacggg 1400
tagggccgcccgccgtggcgcgtcaagccgaagttgccgagcgtgtgccc
caccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacgg 1450
gtggttgcggcgcggctgccgcaagagcgagttgccgtgcgggacgtgcc
tcggctga 1458
agccgact
Forbidden Restriction Sites: PstI x 1.
See below for mutagenic primers.
BIOBRICKTM PRIMERS
Forward: CGT t tctagATG CCT AGG ACC ACG CC
Reverse: CGT ctgcag cggccgc t actagt a TTA TTA GCC GAC CGT GCA GG
MUTAGENIC PRIMERS
781-786 PstI (Gln CAG --> CAA in forward primer)
Forward (786-799): A CGG TTC GCG GAC CTG G (58°C)
Reverse (785-768): TG CAG GTT CTG CCG GAA G (58°C)
