Team:Warsaw/Calendar-Main/9 May 2008

(Difference between revisions)

Latest revision as of 16:32, 28 October 2008


	Attribution analyzer / Interactive list of topics

Restriction digest of pTrc99A-AID with HindIII and NcoI (1x Tango buffer).
DNA electrophoresis and isolation of DNA encoding AID from proper band (600 bp). Fig. 1.
Restriction digest of pMPM-T5 with HindIII and NcoI (1x Tango buffer), dephosphorylation (CIAP).
DNA electrophoresis and isolation of pMPM-T5 vector from proper band (4250 bp). Fig. 2.
Electrophoresis to estimate the concentration of isolated DNA.
Ligation of isolated DNA fragments.

Fig. 1. 1-DNA ladder; 2-pTRC99a-AID digested with NcoI and HindIII

Fig. 2. 1-DNA ladder; 2-pMPM-T5 digested with NcoI and HindIII

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 <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments. </li>
 </ol></p>
-<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/8/89/Gel_out_aid_WAW.jpg" width=300/></a>
+<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/8/89/Gel_out_aid_WAW.jpg" width=170/></a>
 <var><b>Fig. 1.</b> 1-DNA ladder; 2-pTRC99a-AID digested with NcoI and HindIII</var>
-<a name="fig2"><img src="https://static.igem.org/mediawiki/2008/0/0f/Gel_out_pmpm_WAW.jpg" width=300/></a>
+<a name="fig2"><img src="https://static.igem.org/mediawiki/2008/0/0f/Gel_out_pmpm_WAW.jpg" width=200/></a>
 <var><b>Fig. 2.</b> 1-DNA ladder; 2-pMPM-T5 digested with NcoI and HindIII</var>