"
Team:Hawaii/Protocols
From 2008.igem.org
(Difference between revisions)
(→List of Protocols) |
(→E. coli) |
||
| Line 5: | Line 5: | ||
This is a list of tried and true protocols used by our team. | This is a list of tried and true protocols used by our team. | ||
===''E. coli''=== | ===''E. coli''=== | ||
| + | * [[Team:Hawaii/Protocols/Cryopreserve_cells|Cryopreserve cells]] | ||
* [[Team:Hawaii/Protocols/Competent_Cells | Making competent cells]] | * [[Team:Hawaii/Protocols/Competent_Cells | Making competent cells]] | ||
* [[Team:Hawaii/Protocols/Transformation_E_coli|Transformation of '' E. coli'']] | * [[Team:Hawaii/Protocols/Transformation_E_coli|Transformation of '' E. coli'']] | ||
* Alkaline lysis / plasmid isolation | * Alkaline lysis / plasmid isolation | ||
| + | |||
=== ''Synechocystis'' sp. PCC6803=== | === ''Synechocystis'' sp. PCC6803=== | ||
* [[Team:Hawaii/Protocols/Transformation_PCC6803|Triparental conjugation of ''Synechocystis'' sp. PCC6803]] | * [[Team:Hawaii/Protocols/Transformation_PCC6803|Triparental conjugation of ''Synechocystis'' sp. PCC6803]] | ||
Revision as of 22:46, 24 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
List of Protocols
This is a list of tried and true protocols used by our team.
E. coli
- Cryopreserve cells
- Making competent cells
- Transformation of E. coli
- Alkaline lysis / plasmid isolation
Synechocystis sp. PCC6803
Media
- LB
- LB + amp100
- LB + kan20 + amp 50
- LB + sp100
- LB + sp100 + sm50
- BG-11 + 5% LB
- BG-11
- BG-11 + sp2.5 + sm2.5
- Liquid growth media
BioBrick
- [http://openwetware.org/wiki/BioBricks_construction_tutorial BioBrick Construction Tutorial]
- [http://openwetware.org/wiki/Silver:_BB_Strategy Silver:BioBrick Strategy]
- [http://openwetware.org/wiki/Designing_primers#BioBrick_primers Designing BioBrick Primers]
- [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication BioBrick Part Fabrication]
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
