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'''PCR SOEing and Sequencing Primers''' | '''PCR SOEing and Sequencing Primers''' | ||
| - | We created over 100 primers for the construction and sequencing of our plasmids. For the sequences and properties of each primer, as well as which primers were used for which plasmids, | + | We created over 100 primers for the construction and sequencing of our plasmids. For the sequences and properties of each primer, as well as which primers were used for which plasmids, [https://2008.igem.org/Media:PrimerOligos.xls click here]. |
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=== Experiments === | === Experiments === | ||
Revision as of 03:05, 29 October 2008
PRINCETON IGEM 2008
| Home | Project Overview | Project Details | Experiments | Results | Notebook |
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| Parts Submitted to the Registry | Modeling | The Team | Gallery |
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Contents |
Plasmid Designs
We worked on designing and constructing several plasmids this summer. We will shortly put up a list of how far we got with each of them over the course of the summer, and what work remains to be done.
PCR SOEing and Sequencing Primers
We created over 100 primers for the construction and sequencing of our plasmids. For the sequences and properties of each primer, as well as which primers were used for which plasmids, click here.
Experiments
See "Experiments" section
Surface Patterning
It has been shown that plating neurons in a specific pattern gives the emerging networks special properties, which are determined by those patterns. Also, the growth of these networks is strongly influenced by the patterns they are allowed to grow in. Therefore, by patterning the surfaces that neurons grow on, we can direct specific network connections. This is at the heart of the architecture of the toggle switch.
