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Team:Hawaii/Notebook/2008-10-28
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Ran RE digests on gel=== :<strong> Grace</strong> [[Image:102808REdigest.jpg|right|thumb|EtBr stained 4% agarose gel ra...) |
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:* Extracted from gel | :* Extracted from gel | ||
:* Ligated with HindIII/BamHI digested pRL1383a | :* Ligated with HindIII/BamHI digested pRL1383a | ||
| - | :* Used | + | :* Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab) |
| + | ::* Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each. | ||
== Drylab Work == | == Drylab Work == | ||
Revision as of 03:25, 29 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Ran RE digests on gel
- Grace
File:102808REdigest.jpg
EtBr stained 4% agarose gel ran at 60V for 3.5 hours. Ten microliters of XbaI/SpeI digested DNA were loaded into each lane.
- Gel picture for presentation
Antibiotic test
- Grace
File:102808j33207.jpg
EtBr stained 2% agarose gel ran at 60V for 2 hours. Twenty microliters of HindIII/BamHI digested J33207 were loaded.
- Ran HindIII/BamHI digested J33207 on gel
- Extracted from gel
- Ligated with HindIII/BamHI digested pRL1383a
- Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab)
- Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each.
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
