"
Team:Hawaii/Notebook/2008-10-28
From 2008.igem.org
(Difference between revisions)
(→Name of Task) |
(→Project page) |
||
| Line 23: | Line 23: | ||
:* Wrote up Materials, Methods and Results for Project Part B on team project page. | :* Wrote up Materials, Methods and Results for Project Part B on team project page. | ||
| + | ===Sequence analysis=== | ||
| + | :<strong>Grace </strong> | ||
| + | |||
| + | :* Same G->C transversion in CAP binding site for BBpRL-1 sequence. | ||
= Discussion = | = Discussion = | ||
Revision as of 03:35, 29 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Ran RE digests on gel
- Grace
File:102808REdigest.jpg
EtBr stained 4% agarose gel ran at 60V for 3.5 hours. Ten microliters of XbaI/SpeI digested DNA were loaded into each lane.
- Gel picture for presentation
Antibiotic test
- Grace
File:102808j33207.jpg
EtBr stained 2% agarose gel ran at 60V for 2 hours. Twenty microliters of HindIII/BamHI digested J33207 were loaded.
- Ran HindIII/BamHI digested J33207 on gel
- Extracted from gel
- Ligated with HindIII/BamHI digested pRL1383a
- Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab)
- Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each.
Drylab Work
Project page
- Grace
- Wrote up Materials, Methods and Results for Project Part B on team project page.
Sequence analysis
- Grace
- Same G->C transversion in CAP binding site for BBpRL-1 sequence.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
